SOP on Calibration of Polarimeter

 Calibration of Polarimeter

 

1.0 PURPOSE  

The purpose of the standard
operating procedure is to provide guidelines for calibration of 
Polarimeter.

2.0 SCOPE 

The
scope of this standard operating procedure includes calibration of
Polarimeter.

3.0  RESPONSIBILITY

3.1    Section Incharge

3.2    Technical Manager

3.3    Q.A. Manager

4.0 PROCEDURE

4.1
 Ensure that the instrument is clean and
free from dust particles.

4.2  Ensure that the polarimeter tube is cleaned
each time before and after use.

4.3 Switch on the instrument and allow
sufficient time to achieve maximum brightness of the 
sodium lamp.

4.4
Ensure that the settings are done in the zero-zero position.

4.5 Standard
Solution Required For Calibration
:Prepare reference of known
specific rotation 
using Sucrose AR.

4.6
CALIBRATION
:

Open the polarimeter tube compartment and place the
polarimeter tube, which is filled with the standard solution of sucrose in such
a way so as to avoid air bubbles.

The specific rotation observations are done by viewing
through the telescope when the half dark and half light portion of the circle
are in equilibrium.

The half dark and half-light is matched with a knob
rotating it in the clockwise and anti-clockwise directions. It is also defined
as dextro (+) or levo (-) rotating respectively.

A blank determination is also performed and corrections
if any are applied.

Record the corrected observations.

4.7 CALCULATION:

Specific
optical rotation =       (Corrected
observed specific rotation at a specified temp)


                                        (Length of the polarimeter tube in dm) x Conc. of Sucrose soln. %w/w)

Correlate
the observations with the specified values of the various concentrations of
sucrose solutions.

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