SOP on Calibration of Ultra Low Temperature Freezer

1.0 PURPOSE

The purpose of the standard operating procedure is to provide guidelines for the calibration of Ultra-Low Temperature Freezer

2.0 SCOPE

The scope of this standard operating procedure includes the calibration of Ultra-Low Temperature Freezer

3.0 RESPONSIBILITY

3.1 Q.A. Executive

3.2 Technical Manager

3.3 Q.A. Manager

4.0 REQUIREMENTS

4.1 Calibrated Digital Temperature (-80 to 199.9 ⁰C)

4.2 Instrument Instruction Manual

5.0 PROCEDURE

5.1 Calibration Procedure

5.1.1 Set -80 ⁰C temperature at which temperature calibration of Ultra –Low Temperature freezer has to be done.

5.1.2 Use the set key & press it on the control panel of the Ultra –Low Temperature to memorise the temperature.

Press the numerical Value Shift Key & digital shift key to set -80 ⁰C temperature.
Wait for the set temperature to flash on control panel display screen.
Wait for few minutes to maintain the temperature in Ultra –Low Temperature freezer.

5.1.6 Open the door latch & insert the PT-100 sensor of calibrated digital thermometer (-80 to 199.9 ⁰C)

Record the temperature observed on Ultra-Low Temperature freezer & calibrated digital thermometer.

5.1.7 Record the temperature at set temperature at different time interval first reading at zero hr, Second reading at the interval of 2 hrs & third reading after 2 hrs of second reading.

5.1.8 Zero hr will be defined as the time of starting the calibration of Ultra Low Temperature Freezer.

5.2 Calibration Record

Sr.No	Calibration Time Intervals	Temperature UUC (⁰C)	Temperature Reference (⁰C)	Correction to UUC In (⁰C) if required
1	0 hr	-80 ⁰C
2	0- 2 hr	-80 ⁰C
3	0- 4 hr	-80 ⁰C