COLUMN MANAGEMENT SYSTEM

COLUMN MANAGEMENT SYSTEM

In this PPT we cover fallowing Topic…..

1.0 Introduction & background

2.0 Column chemistry – shape and purification of silica/effect of particle size/reverse phase & normal phase (ODS/BDS/CN/Phenyl/amino/end capping/carbon loading/effect of inorganic impurities-chelating /, length, inner diameter, polarity & viscosity of solvents)

3.0 Common Terminology

4.0 Types of Column – As per IP, BP & USP-NF

5.0 Different manufacturers / Brands of column

6.0 Column receipt, performance check & procedure for keeping in routine use

7.0 Handling precautions (Care, maintenance and Storage of Column)

8.0 Factors effecting system pressure

9.0 Guard column

10.0 Problems arising in HPLC column and corrective action.

11.0 Regeneration

COLUMN MANAGEMENT SYSTEM PPT

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