Source, Method of
Production, Chemical Constituents, Chemical Tests, Uses and Adulterants of Honey
Objectives
At the end of this
lecture, student will be able to:
• Discuss the pharmacognostical aspects of
– Honey
Honey
Synonym: Mel,
Madhu, Honey purified
Source: Sugary
secretion deposited in the honey combs by the bees Apis mellifera, Apis dorsata
and other species of Apis
Family: Apidae
Preparation/Collection
• Nectar (25% sucrose, 75 % water)
• Invertase
• Smoked
• Centrifugation
• Natural draining
• Apply pressure
• Heated to 80oC
• Rapidly cooled
Description
• Colour: Pale yellow to yellowish brown
• Odour: Pleasant and characteristic
• Taste: Sweet and faintly acrid
Chemical constituents
• 70-80% of invert sugar, mixture of dextrose and levulose
• Glucose, fructose, sucrose
• Acids – formic acid, acetic acid, succinic acid
• Enzymes – Invertase, diastase, inulase
• Vitamins, proteins, pollen grains
Chemical tests
• 1 ml of honey + 4 ml of alcohol, some more honey:
– At 4 ml the solution is clear, upon addition of honey, the
solution does not becomes turbid. (Turbidity shows the presence of dextrin and
added glucose)
• Fiehe’s test
Uses
• Demulcent
• Sweetening agent
• Antiseptic for
burns and wounds
• Preparation of
creams, lotions, soft drinks and candies
• Vehicle for
ayurvedic preparations
Substitutes and
adulterants
• Artificial invert sugar
• Sucrose
• Commercial glucose
• Liquid glucose
• Adulterants normally contain large amounts of furfural
derivatives – Fiehe’s test
– Honey – extracted with ether, ethereal layer – separated,
dried
– To the residue 1% resorcinol in hydrochloric acid is added
– Faint pink colour – natural
– Persistent deep cherry red / pink colour – adulterated
Summary
• Honey is the sugary secretion from the honey comb with
invert sugar content
• Adulterant in honey can be identified by Fiehes test