SOP for calibration of Auto Titrator
1.0
PURPOSE
The purpose of the standard operating procedure is
to provide guidelines for calibration of Auto Titrator.
to provide guidelines for calibration of Auto Titrator.
2.0 SCOPE
The
scope of this standard operating procedure includes calibration of Auto Titrator.
scope of this standard operating procedure includes calibration of Auto Titrator.
3.0 RESPONSIBILITY
3.1 Section In charge
3.2 Technical Manager
3.3 Q.A. Manager
4.0 PROCEDURE
4.1 DETERMINATION OF VOLUME:
4.1.1 Clean the measuring / dispensing
pipette available with the respective set of the,
pipette available with the respective set of the,
equipment.
4.1.2 Fill the measuring pipette with distilled
water and adjust to the zero point.
water and adjust to the zero point.
4.1.3
Dispense the known volumes of water at random or from the set point of
the
Dispense the known volumes of water at random or from the set point of
the
graduation marks of the pipette.
4.1.4
Repeat the dispensing of water at different set points and weigh the
beaker again
Repeat the dispensing of water at different set points and weigh the
beaker again
and again.
4.1.5
From the different weights obtained
calculate the volume dispensed and co-relate
From the different weights obtained
calculate the volume dispensed and co-relate
with
the graduation of the pipette and assign the value.
the graduation of the pipette and assign the value.
4.2 CALCULATION:
Weight of the water dispensed (gm)
Volume of the pipette (ml), V= ————————————————
Density of Water at 27oC (gm / ml)
Where,
D = Density (gm/ml) of Water at 27oC