SOP for Calibration of Antibiotic Zone Reader (Projection Type)

SOP on Calibration of Antibiotic Zone Reader 

(Projection Type)

1.0 PURPOSE

The purpose of the standard
operating procedure is to provide guidelines for calibration of 
AntibioticZone Reader
(Projection Type)
2.0 SCOPE
  

   The
scope of this standard operating procedure includes calibration of
Antibiotic
Zone

    Reader
(Projection Type)

3.0 RESPONSIBILITY

3.1    Q.A. Executive

3.2    Technical-Head (Microbiology Dept)

3.3    Q.A. Manager

4.0 REQUIREMENTS

4.1
Calibrated Vernier Caliper

4.2  
Calibrated coins of different diameters

4.2.1 
Calibrated coin A (diameter-21.98 mm)

4.2.2 
Calibrated coin B (diameter-24.92 mm)

4.2.3 
Calibrated coin C (diameter-26.76 mm)

4.3  Antibiotic Zone Reader (Projection Type)

5.0   PROCEDURE

5.1    Calibration Procedure:                                                                                             

5.1.1     Check the power supply & on the Antibiotic
Zone Reader

5.1.2     Adjust the light intensity of Antibiotic Zone
Reader

5.1.3     Place the calibrated coin A at the centre
of projection scale of Antibiotic Zone Reader.

 

 
5.1.4     Measure the projection Zone of Antibiotic
Zone Reader in mm .Repeat it at least three times &  

              take average & record it.

5.1.5     
Place the Calibrated coin B at the centre of
projection scale of Antibiotic Zone Reader.

5.1.6     
Repeat the step 5.1.6 & record the
diameter of projection scale using coin B in mm.

5.1.7     
 Place
the Calibrated coin C at the centre of projection scale of Antibiotic Zone
Reader .

5.1.8     
 Repeat
the step 5.1.6 & record the diameter of projection scale using coin C in
mm.

                                                           
                                                                     

5.2.       Calibration
Record

Sr.No

Calibrated Coin

Reading of calibrated coins in mm

Reading of
Projection Zone (mm)

Reading of
Projection Zone (mm) in Average

I  

II

III

 
   1

    Coin A

     2

    Coin B

     3

    Coin C

Calibrated 
By                                                                              
                             Checked
By

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