SOP FOR CLEANING OF GLASSWARE USED IN QUALITY CONTROL LABORATORY
SOP FOR CLEANING OF GLASSWARE USED IN QUALITY CONTROL LABORATORY
1.0 OBJECTIVE
1.1 To lay down a standard procedure for cleaning of glassware used in Quality Control Laboratory.
2.0 SCOPE
2.1 This procedure is applicable for cleaning of glassware used in Quality Control Laboratory.
3.0 RESPONSIBILITY
3.1 Executive/Officer – QC.
4.0 ACCOUNTABILITY
4.1 Head -Quality Control.
5.0 PROCEDURE
5.1 General Glassware
5.1.1 The used glassware shall be kept in the designated plastic container labeled as ´Used Glassware for cleaning.
5.1.2 Used Glassware shall be washed with plenty of raw water.
5.1.3 Glassware shall be scrubbed adding soap solution and washed properly with raw water till the soap solution is removed.
5.1.4 Subsequently glassware shall be rinsed with purified water.
5.1.5 Finally the Glassware shall be rinsed with Acetone and dried at 40 0C.
5.1.6 After drying the Glassware shall be removed and stored in their designated places.
5.1.7 If any stains or other contamination observed in the glass ware, pipettes, burettes & other glassware shall be soaked in
hot dilute Nitric Acid for 4 hours to remove stains or other contamination in the glassware.
5.1.8 Finally the Nitric Acid treated pipettes, burettes and other glassware shall be rinsed with Purified water followed by Acetone and dried at 40 ºC .
5.2 Pipettes & Burettes
5.2.1 Pipettes and burettes shall be flushed with running water under the tap.
5.2.2 At least half of the burette or pipette (by sucking with bulb) shall be filled with the soap solution and rinsed thoroughly by rotating the pipette or burette.
5.2.3 Wash properly with raw water till the soap solution is removed.
5.2.4 Subsequently follow the same steps as in 5.1.3 to 5.1.7
5.3 HPLC Vials (auto sampler)
5.3.1 Glassware such as auto sampler vials shall be first soaked in raw water, followed by soaking in soap solution.
5.3.2 Subsequently glassware shall be rinsed with purified water.
5.3.3 Finally the Glassware shall be rinsed with Methanol and dried at 40 ºC.
5.3.4 After drying the Glassware shall be removed and stored in their designated places.
5.4 GC Vials
5.4.1 After the completion of analysis de – crimp the vials and discard the solution.
5.4.2 Follow the same steps as in 5.3.1 to 5.3.2.
5.4.3 Collect about 800 ml of purified water in a 1000 ml beaker and immerse the vials in the water.
5.4.4 Heat the beaker on a heating mantle, till the water starts boiling. Continue boiling for one hour.
5.4.5 After one hour of boiling, discard the water from the beaker and the vials.
5.4.6 Dry the vials in a hot air oven at about 110 ºC, keeping them in inverted position in a stand.
5.4.7 Cool the dried vials and transfer them to a poly bag and label the poly bag as `Cleaned vials for ready use , sign and date.
6.0 ENCLOSURES
6.1 Nil
7.0 ABBREVIATIONS
7.1 SOP Standard Operating Procedure
7.2 Q.C. Quality Control
7.3 0C – Degree Celsius.
8.0 REFERENCES
8.1 In house
END OF DOCUMENTS
Also, Visit:
B. Pharma Notes | B. Pharma Notes | Study material Bachelor of Pharmacy pdf