Calibration of BOD Incubator

1.0      PURPOSE

            The purpose of the standard
operating procedure is to provide guidelines for calibration of BOD Incubator.

2.0    SCOPE    

           The scope of this standard operating procedure includes calibration of
BOD Incubator.

3.0   RESPONSIBILITY

3.1    Section Incharge

3.2    Technical Manager

3.3    Q.A. Manager

4.0   PROCEDURE

4.1   Switch on the instrument.

           
4.2  Ensure that the temperature
indicator / controller is in working condition i.e. the set

       
temperature should be maintained/retained properly.

4.3   Ensure that the door of incubator is properly
closed and does not loosen during

        operation.

4.4   Ascertain and set the points/points at which
calibration is required.

4.5  Switch on the incubator and allow it to reach
the set point temperature.

4.6  Apparatus Required For Calibration:
Platinum resistance thermometer  with
sensor and

      
digital temperature indicator.

4.7  CALIBRATION:

4.7.1 Insert the sensor of the
reference/calibrator sensor (PRT) in the BOD incubator as near as possible to
the sensing tip or sensor of the temperature controller/indicator of the BOD
incubator.

4.7.2 
When the temperature is stable note down the temperature reading as
indicated by

          the BOD incubator and the reference
indicator simultaneously.

4.7.3     Set
the next point and record the temperature readings accordingly.

4.7.4
   Take at least three to four readings
for better accuracy and reproducibility.

4.7.5    Correlate
the readings observed with the reference standards and apply corrections, if
necessary.