Calibration of Gas Chromatograph(GC)

1.0    PURPOSE  

         The purpose of the standard
operating procedure is to define a method to calibrate the

         Gas Chromatography (GC).

2.0
   SCOPE:   

         This standard
operating procedure is applicable to the staff responsible for operation

         and calibration of the Gas
Chromatography (GC).

3.0    RESPONSIBILITY:

3.1    Section Incharge

3.2    Technical Manager

3.3    Q.A. Manager 

4.0      PROCEDURE:

4.1      MATERIALS REQUIRED:

4.1.1
  Instrument
to be used

4.1.2    Hydrogen
Cylinder

4.1.3    Air
Cylinder

4.1.4    Calibrated
Temperature Probe

4.1.5    Ethanol

4.1.6    Glassware

4.2      FLOW VERIFICATION:

Using
the standard operating conditions start the carrier gas flow through at a set

point.

Allow
the gas to pass through the gas flow meter supplied with the instrument and
record the gas flow rate.

Similarly
allow the hydrogen gas and air gas to pass through at a set point flow rate of
30ml/minute and 300ml/minute respectively. The gases are allowed to pass
through the gas flow meter and the flow rates recorded.

Correlate
the observed gas flow rates with the set point flow rates and make adjustments,
if necessary. 

4.3   TEMPERATURE VERIFICATION:

Insert
a calibrated temperature probe into the oven near the oven sensor. Allow all
zones to stabilize and the to become ready. Record the actual temperature probe
value for oven temperature.

4.4    Detector/Auto sampler Reproducibility

4.4.1
Repeatability (Precision)

Repeatability
will look at the standard deviation of 6 injections of the same concentration
of the 0.5% solution of ethanol. From the standard deviation the RSD is
calculated. If the standard deviation is less than or equal to 2.0 % the test
passes. The purpose of Repeatability is to verify that the Autosampler can
repeatedly inject the same volume of sample numerous times. Inject 6 injections
of 1mL
each.

4.4.2
LINEARITY

Linearity
will check that the Autosampler can accurately inject different concentration
of ethanol. We do this by injecting multiple samples of different concentrations.
The injection volume is 1mL
from 0.40%, 0.45%, 0.50%, 0.55% and 0.6 % w/v solution of ethanol. There should
be linear be a linear relationship between sample concentration and area under
the peak.