SOP on Calibration of Infrared Spectrophotometer

 Calibration of Infrared Spectrophotometer

 

1.0  PURPOSE 
   The purpose
of the standard operating procedure is to provide guidelines for calibration of
Infrared Spectrophotometer.

2.0  SCOPE   

  
The scope of this standard operating procedure
includes calibration of
Infrared

    Spectrophotometer.

3.0 RESPONSIBILITY

3.1    Section Incharge

3.2    Technical Manager

3.3    Q.A. Manager

4.0 PROCEDURE

Infra red spectrophotometer is a technique used for
identifying the organic compounds.

Control the humidity of the environment within the range
of 40 to 50% RH at 25oC.

Switch on the instrument and observe that it initialises
at 4018
±
1cm-1.

 

4.1
Standard Required For Calibration
:

       Standard Polystyrene Film

4.2
Calibration
:

Using the stipulated operating conditions run in the
infra-red spectra of the reference polystyrene film supplied with the
instrument.  

All the significant or principal peaks in the spectrum
obtained should be at the same wavelength as that of the standard reference
graph and should correspond to within 0.5% of the wave number scale. The
relative sizes of the bands should be concordant in the two spectra.

4.3 FREQUENCY

         At every 3 months

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