SOP on Calibration of Inductively Coupled Plasma Mass Spectrometer

 Calibration
of Inductively Coupled 
Plasma Mass Spectrometer

1.0 PURPOSE   

The purpose of the standard operating procedure is
to provide guidelines for calibration of

      Inductively Coupled Plasma Mass
Spectrometer.

2.0 SCOPE  

      The
scope of this standard operating procedure includes calibration of Inductively
Coupled

       Plasma Mass Spectrometer.

3.0   RESPONSIBILITY

3.1   Application Scientist

3.2   Technical-Manager

3.3    Q.A. Manager

4.0 REQUIREMENTS

4.1
Argon Gas Cylinders

4.2  
Elan 9000 Hardware Guide

4.3  
Elan Inductively Coupled Plasma Mass
Spectroscopy safety manual

4.4  
Tuning Solution

5.0   PROCEDURE

5.1 Precaution while start-up the
Inductivel Coupled Plasma Mass Spectrometer

5.1.1  Argon gas cylinder should not be used below
100 psi in term of gas quantity

5.1.2 
 Release
gas pressure should be between 60-80 psi.

5.2 
Switch on the blower & recirculater.

5.3 
Before the ignition of the plasma, vacuum
pressure should be 10-6 torr.

5.4 
Open Software.           

5.4.1  Open “Instrument “———Start Plasma

5.4.1  Leave
the plasma for 30 minutes or more (minimum 25 minutes).

5.4.2     
Open —-“ Workspace” ———-“Daily
Performance .wrk”

5.4.3     
Place the tuning solution.

5.4.4     
Analyse the above solution as “Sample “.

5.4.5     
Do  Daily
Performance Check

5.4.5.1 
Mg intensity > 40,000 cps/10 ppb

5.4.5.2 
In Intensity > 250 , 000 cps/10 ppb

5.4.5.3 
U intensity > 200,000 cps/10 ppb

5.4.5.4 
CeO/Ce ;Ba++/Ba < 3 %

5.4.5.5 
Background 220 <2 cps

5.4.6      If
above test is not OK then optimize. Optimisation is required only when the
value are highly disturbed .Do optimization while referring to the manual.
following parameters must be optimized.
 

5.4.6.1 
Nebuliser Gas Flow (NEB)

5.4.6.2 
Auxiliary Gas Flow

5.4.6.3 
Plasma Gas Flow 

5.4.6.4 
Lens Voltage

5.4.6.5 
Inductively Coupled Plasma Radio-frequency
(Rf) power
 

5.4.6.6 
Analog Stage voltage

5.4.6.7 
Pulse Stage voltage 

5.4.6.8 
Quadropole Rod Offset (QRD) 

5.4.6.9 
Cell Path Voltage (CPV) 

5.4.7     
Click on get Analyte list Icon & select
Analyte from Analyte Icon i.e.Mg
 

5.4.8     
Select optimization criteria & select
maximum intensity.

5.4.9     
Click on optimize icon. 

5.4.10  
Repeat the step 5.4.8 , 5.4.9 & 5.4.10  for above mentioned parameters individually i.e.
Nebuliser

             Gas Flow, Auxiliary Gas Flow, and Plasma
Gas flow

6.0    REFERENCE

6.1   Working Instructions (Inductively Coupled
Plasma Mass Spectrometer); Issue date: Jan  2006

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