Calibration of Karl Fischer Apparatus
SOP on Calibration of Karl Fischer Apparatus
1.0 PURPOSE
The purpose of the standard operating procedure is to provide guidelines for calibration of Karl Fischer Apparatus..
2.0 SCOPE
The scope of this standard operating procedure includes calibration of Karl Fischer Apparatus.
3.0 RESPONSIBILITY
3.1 Application Scientist
3.2 QA Manager
3.3 Director Technical
4.0 PROCEDURE
4.1 APPARATUS REQUIRED FOR CALIBRATION:
4.1.1 Dried Beakers, which have been previously rinsed with acetone.
4.1.2 Calibrated Analytical Balance
4.2 DETERMINATION OF VOLUME:
4.2.1 Clean the measurement/dispensing pipette available with the respective set of the equipment.
4.2.2 Fill the measuring pipette with water and adjust to the zero point.
4.2.3 Dispense the known volumes of water at random or from the set point of the graduation marks of the pipette.
4.2.4 Repeat the dispensing of water at different set points and weigh the beaker again and again.
4.2.5 From the different weights obtained calculate the volume dispensed and co-relate with the graduation of the pipette and assign the value.
4.3 CALCULATION:
Weight of the water dispensed (gm)
Volume of the pipette (ml), V= ————————————————–
Density of Water at 27oC (gm/ml)
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