SOP on Calibration of Photofluorimeter

 Calibration of Photofluorimeter

1.0 PURPOSE   

      The
purpose of the standard operating procedure is to provide guidelines for
calibration of

      
Photofluorimeter.

2.0 SCOPE  

      The scope of this
standard operating procedure includes calibration of
Photofluorimeter.

3.0  RESPONSIBILITY

3.1  Section In charge

3.2  Technical Manager

3.3  Q.A. Manager

4.0  
PROCEDURE

4.1   On the instrument 15 minutes before starting
the calibration.

4.2   Make dilutions using fluoroscene in 0.1 M NaOH
(1ppm, 2ppm, 3ppm and 4 ppm).

4.3   Use 0.1M NaOH as blank and set the instrument
on “0 “with blank knob.

4.4   Set the instrument at ‘20’ with 1ppm solution.

4.5   Take the readings at 1, 2, 3 and 4 ppm without
touching any of the knobs.

4.6   Standard readings for above mentioned
concentrations:

 

S.
No.

Concentration(ppm)

Observed
Intensity(Units)

1

1
ppm

21

2

2
ppm

40

3

3
ppm

60

4

4
ppm

79

 

  

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