Different methods of analysis – Pharmaceutical Analysis 1 B. Pharma 1st semester

Different methods of analysis

Content

• The different types of Titrimetric analysis

• Apparatus used for titration process

• Different methods of volumetric titration

Learning Objectives

At the end of this
lecture, the student will be able to

• Define and classify the different types of Titrimetric
analysis

• List of apparatus used for titration process

• List the methods for expressing concentrations in
volumetric analysis

• Explain different methods of volumetric titration

Analytical
Chemistry

– Analytical chemistry deals with separating, identifying,
and quantifying the relative amounts of the components of an analyte.

– Analyte = the thing to analyzed; the component(s) of a sample
that are to be determined

The role of analytical chemistry: central science

The relationship between analytical chemistry and the other
sciences

Analytical chemistry

Chemistry:  
Biological, Inorganic, Organic, Physical

Physics: Astrophysics, Astronomy, Biophysics

Biology: Botany, Genetics, Microbiology, Molecular biology,
Zoology

Geology: Geophysics, Geochemistry, Paleontology,
Paleobiology

Environmental science: Ecology, Meteorology, Oceanography

Medicine: Clinical, Medicinal, Pharmacy, Toxicology

Material science: Metallurgy, Polymers, Solid state

Engineering: Civil, Chemical, Electronical, and Mechanical

Agriculture: Agronomy, Animal, Crop, Food, Horticulture,
Soil

Social Science: Archeology, Anthropology, Forensics

Pharmaceutical
analysis and its scope

1. Clinical analysis – Blood, urine, feces, cellular fluids,
etc., for use in diagnosis.

2. Pharmaceutical analysis – Establish the physical
properties, toxicity, metabolites, quality control, etc.

3. Environmental analysis – Pollutants, soil and water
analysis, pesticides.

4. Forensic analysis – Analysis related to criminology; DNA
finger printing, finger print detection; blood analysis.

5. Industrial quality control – Required by most companies
to control product quality.

6. Bio analytical chemistry and analysis – Detection and/or
analysis of biological components (i.e., proteins, DNA, RNA, carbohydrates,
metabolites, etc.). This often overlaps many areas.   Develop new tools for basic and clinical research.

Classification
of Analytical Chemistry

Qualitative analysis

What is present?

-Determination of chemical identity of the species in the
sample.

Quantitative analysis

How much present?

Determination of the amount of species or analytes, in
numerical terms

Classification
of Quantitative Analysis

Chemical Methods

Electrical Methods

Instrumental methods

Biological and microbiological

1. Chemical
Methods

Volumetric or titrimetric methods                                 

Gasometric
analysis 

Gravimetric
methods

2.
Electrical Methods


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